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genechip whole transcript (wt) expression arrays  (Thermo Fisher)


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    Thermo Fisher genechip whole transcript (wt) expression arrays
    Genechip Whole Transcript (Wt) Expression Arrays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genechip+whole+transcript+(wt)+expression/genechip+whole+transcript++wt++plus+reagent+kit/pm39357526-54-18-24
    Average 90 stars, based on 1 article reviews
    genechip whole transcript (wt) expression arrays - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Identification of autosomal cis expression quantitative trait methylation (cis eQTMs) in children’s blood
    Article Snippet: .. Affymetrix HTA 2.0 arrays were hybridized according to Affymetrix recommendations using the Manual Target preparation for GeneChip Whole Transcript (WT) expression arrays and the labeling and hybridization kits. ..

    Article Title: Comparative Screening of the Liver Gene Expression Profiles from Type 1 and Type 2 Diabetes Rat Models
    Article Snippet: The samples were processed and hybridized to Affymetrix’s Clariom TM D Rat microarray chips (Affymetrix, Santa Clara, CA, USA) in the Microarray Unit of the National Institute of Genomic Medicine (INMEGEN) in Mexico. .. The hybridization and scanning of the microarray were performed according to GeneChip TM Whole Transcript (WT) Expression Arrays User Guide of Affymetrix Corporation. ..

    Article Title: Neurodevelopmental alcohol exposure elicits long-term changes to gene expression that alter distinct molecular pathways dependent on timing of exposure
    Article Snippet: All RNA labeling and hybridization steps were performed at the London Regional Genomics Centre (Robarts Research Institute, London, ON, Canada). .. Briefly, single-stranded complementary DNA (sscDNA) was synthesized using 200 ng of total RNA using the Ambion WT Expression Kit for Affymetrix GeneChip Whole Transcript WT Expression Arrays (Applied Biosystems, Carlsbad, CA, USA) and the Affymetrix GeneChip WT Terminal Labeling kit and hybridization manual (Affymetrix, Santa Clara, CA, USA). .. First-cycle cDNA was transcribed in vitro to cRNA, and used to synthesize 5.5 μg of sscDNA that was subsequently end-labeled and hybridized for 16 h at 45°C to Affymetrix Mouse Gene 1.0 ST arrays.

    Article Title: Distinct Effects of Mitochondrial Na <sup>+</sup> /Ca <sup>2+</sup> Exchanger Inhibition and Ca <sup>2+</sup> Uniporter Activation on Ca <sup>2+</sup> Sparks and Arrhythmogenesis in Diabetic Rats
    Article Snippet: .. Briefly, tissue RNA was labeled using Ambion WT expression kit (for use with Affymetrix GeneChip Whole Transcript Expression Arrays MAN0018137 revision). ..

    Article Title: Mechanical Tension Increases CCN2/CTGF Expression and Proliferation in Gingival Fibroblasts via a TGFβ-Dependent Mechanism
    Article Snippet: RNA quality was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Palo Alto, CA) and the RNA 6000 Nano kit (Caliper Life Sciences, Mountain View, CA). .. Single stranded complimentary DNA (sscDNA) was prepared from 200 ng of total RNA as per the Ambion WT Expression Kit for Affymetrix GeneChip Whole Transcript WT Expression Arrays ( http://www.ambion.com/techlib/prot/fm_4411973.pdf , Applied Biosystems, Carlsbad, CA) and the Affymetrix GeneChip WT Terminal Labeling kit and Hybridization User Manual ( http://media.affymetrix.com/support/downloads/manuals/wt_term_label_ambion_user_manual.pdf , Affymetrix, Santa Clara, CA). ..

    Labeling:

    Article Title: Identification of autosomal cis expression quantitative trait methylation (cis eQTMs) in children’s blood
    Article Snippet: .. Affymetrix HTA 2.0 arrays were hybridized according to Affymetrix recommendations using the Manual Target preparation for GeneChip Whole Transcript (WT) expression arrays and the labeling and hybridization kits. ..

    Article Title: Neurodevelopmental alcohol exposure elicits long-term changes to gene expression that alter distinct molecular pathways dependent on timing of exposure
    Article Snippet: All RNA labeling and hybridization steps were performed at the London Regional Genomics Centre (Robarts Research Institute, London, ON, Canada). .. Briefly, single-stranded complementary DNA (sscDNA) was synthesized using 200 ng of total RNA using the Ambion WT Expression Kit for Affymetrix GeneChip Whole Transcript WT Expression Arrays (Applied Biosystems, Carlsbad, CA, USA) and the Affymetrix GeneChip WT Terminal Labeling kit and hybridization manual (Affymetrix, Santa Clara, CA, USA). .. First-cycle cDNA was transcribed in vitro to cRNA, and used to synthesize 5.5 μg of sscDNA that was subsequently end-labeled and hybridized for 16 h at 45°C to Affymetrix Mouse Gene 1.0 ST arrays.

    Article Title: Distinct Effects of Mitochondrial Na <sup>+</sup> /Ca <sup>2+</sup> Exchanger Inhibition and Ca <sup>2+</sup> Uniporter Activation on Ca <sup>2+</sup> Sparks and Arrhythmogenesis in Diabetic Rats
    Article Snippet: .. Briefly, tissue RNA was labeled using Ambion WT expression kit (for use with Affymetrix GeneChip Whole Transcript Expression Arrays MAN0018137 revision). ..

    Article Title: Mechanical Tension Increases CCN2/CTGF Expression and Proliferation in Gingival Fibroblasts via a TGFβ-Dependent Mechanism
    Article Snippet: RNA quality was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Palo Alto, CA) and the RNA 6000 Nano kit (Caliper Life Sciences, Mountain View, CA). .. Single stranded complimentary DNA (sscDNA) was prepared from 200 ng of total RNA as per the Ambion WT Expression Kit for Affymetrix GeneChip Whole Transcript WT Expression Arrays ( http://www.ambion.com/techlib/prot/fm_4411973.pdf , Applied Biosystems, Carlsbad, CA) and the Affymetrix GeneChip WT Terminal Labeling kit and Hybridization User Manual ( http://media.affymetrix.com/support/downloads/manuals/wt_term_label_ambion_user_manual.pdf , Affymetrix, Santa Clara, CA). ..

    Hybridization:

    Article Title: Identification of autosomal cis expression quantitative trait methylation (cis eQTMs) in children’s blood
    Article Snippet: .. Affymetrix HTA 2.0 arrays were hybridized according to Affymetrix recommendations using the Manual Target preparation for GeneChip Whole Transcript (WT) expression arrays and the labeling and hybridization kits. ..

    Article Title: Comparative Screening of the Liver Gene Expression Profiles from Type 1 and Type 2 Diabetes Rat Models
    Article Snippet: The samples were processed and hybridized to Affymetrix’s Clariom TM D Rat microarray chips (Affymetrix, Santa Clara, CA, USA) in the Microarray Unit of the National Institute of Genomic Medicine (INMEGEN) in Mexico. .. The hybridization and scanning of the microarray were performed according to GeneChip TM Whole Transcript (WT) Expression Arrays User Guide of Affymetrix Corporation. ..

    Article Title: Neurodevelopmental alcohol exposure elicits long-term changes to gene expression that alter distinct molecular pathways dependent on timing of exposure
    Article Snippet: All RNA labeling and hybridization steps were performed at the London Regional Genomics Centre (Robarts Research Institute, London, ON, Canada). .. Briefly, single-stranded complementary DNA (sscDNA) was synthesized using 200 ng of total RNA using the Ambion WT Expression Kit for Affymetrix GeneChip Whole Transcript WT Expression Arrays (Applied Biosystems, Carlsbad, CA, USA) and the Affymetrix GeneChip WT Terminal Labeling kit and hybridization manual (Affymetrix, Santa Clara, CA, USA). .. First-cycle cDNA was transcribed in vitro to cRNA, and used to synthesize 5.5 μg of sscDNA that was subsequently end-labeled and hybridized for 16 h at 45°C to Affymetrix Mouse Gene 1.0 ST arrays.

    Article Title: Mechanical Tension Increases CCN2/CTGF Expression and Proliferation in Gingival Fibroblasts via a TGFβ-Dependent Mechanism
    Article Snippet: RNA quality was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Palo Alto, CA) and the RNA 6000 Nano kit (Caliper Life Sciences, Mountain View, CA). .. Single stranded complimentary DNA (sscDNA) was prepared from 200 ng of total RNA as per the Ambion WT Expression Kit for Affymetrix GeneChip Whole Transcript WT Expression Arrays ( http://www.ambion.com/techlib/prot/fm_4411973.pdf , Applied Biosystems, Carlsbad, CA) and the Affymetrix GeneChip WT Terminal Labeling kit and Hybridization User Manual ( http://media.affymetrix.com/support/downloads/manuals/wt_term_label_ambion_user_manual.pdf , Affymetrix, Santa Clara, CA). ..

    other:

    Article Title: Application of Gene Expression Microarray for the Classification of Ph-Like B-Cell Acute Lymphoblastic Leukemia.
    Article Snippet: Introduction: Phlike ALL has gene expression profile similar to Phpositive ALL but without the BCR::ABL1 fusion.. The disease presents higher rates of severe clinical features and is associated with unfavorable outcomes.. There is still no standard pipeline for molecular characterization of the disease, and no valid predictor gene panel is available worldwide.

    Article Title: The Human Placental Sexome Differs between Trophoblast Epithelium and Villous Vessel Endothelium
    Article Snippet: Total RNA was labeled using Ambion WT Expression Kit for Affymetrix GeneChip Whole transcript (WT) Expression Arrays (Life Technologies; Carlsbad, CA, USA).

    Microarray:

    Article Title: Comparative Screening of the Liver Gene Expression Profiles from Type 1 and Type 2 Diabetes Rat Models
    Article Snippet: The samples were processed and hybridized to Affymetrix’s Clariom TM D Rat microarray chips (Affymetrix, Santa Clara, CA, USA) in the Microarray Unit of the National Institute of Genomic Medicine (INMEGEN) in Mexico. .. The hybridization and scanning of the microarray were performed according to GeneChip TM Whole Transcript (WT) Expression Arrays User Guide of Affymetrix Corporation. ..

    Synthesized:

    Article Title: Neurodevelopmental alcohol exposure elicits long-term changes to gene expression that alter distinct molecular pathways dependent on timing of exposure
    Article Snippet: All RNA labeling and hybridization steps were performed at the London Regional Genomics Centre (Robarts Research Institute, London, ON, Canada). .. Briefly, single-stranded complementary DNA (sscDNA) was synthesized using 200 ng of total RNA using the Ambion WT Expression Kit for Affymetrix GeneChip Whole Transcript WT Expression Arrays (Applied Biosystems, Carlsbad, CA, USA) and the Affymetrix GeneChip WT Terminal Labeling kit and hybridization manual (Affymetrix, Santa Clara, CA, USA). .. First-cycle cDNA was transcribed in vitro to cRNA, and used to synthesize 5.5 μg of sscDNA that was subsequently end-labeled and hybridized for 16 h at 45°C to Affymetrix Mouse Gene 1.0 ST arrays.



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    Experimental design applied to identify sex-biased gene expression in four placental cell types: syncytiotrophoblast (SCT), cytotrophoblasts (CT), arterial (AEC) and venous endothelial cells (VEC), two main placental compartments (trophoblast epithelium and villous vessel endothelium), and in the whole placental villi. The cells were isolated from placentas of male and female fetuses and their mRNA subjected to microarray analysis by hybridization to Affymetrix GeneChip Human 1.0 ST arrays. Genes that reached significance (p<0.05) and with a fold-change >1.3 were considered differentially expressed between the two sexes. Placental villi refers to the combined analysis of all expressed transcripts in syncytiotrophoblast, cytotrophoblasts, arterial and venous endothelial cells comparing gene expression in male vs. female cells. Microarray data were validated by RT-qPCR a) using identical samples as used in the microarray analysis and b) in different isolations cultured independently.

    Journal: PLoS ONE

    Article Title: The Human Placental Sexome Differs between Trophoblast Epithelium and Villous Vessel Endothelium

    doi: 10.1371/journal.pone.0079233

    Figure Lengend Snippet: Experimental design applied to identify sex-biased gene expression in four placental cell types: syncytiotrophoblast (SCT), cytotrophoblasts (CT), arterial (AEC) and venous endothelial cells (VEC), two main placental compartments (trophoblast epithelium and villous vessel endothelium), and in the whole placental villi. The cells were isolated from placentas of male and female fetuses and their mRNA subjected to microarray analysis by hybridization to Affymetrix GeneChip Human 1.0 ST arrays. Genes that reached significance (p<0.05) and with a fold-change >1.3 were considered differentially expressed between the two sexes. Placental villi refers to the combined analysis of all expressed transcripts in syncytiotrophoblast, cytotrophoblasts, arterial and venous endothelial cells comparing gene expression in male vs. female cells. Microarray data were validated by RT-qPCR a) using identical samples as used in the microarray analysis and b) in different isolations cultured independently.

    Article Snippet: Total RNA was labeled using Ambion WT Expression Kit for Affymetrix GeneChip Whole transcript (WT) Expression Arrays (Life Technologies; Carlsbad, CA, USA).

    Techniques: Expressing, Isolation, Microarray, Hybridization, Quantitative RT-PCR, Cell Culture